Banca de QUALIFICAÇÃO: SWANNY FERREIRA BORGES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : SWANNY FERREIRA BORGES
DATE: 06/05/2025
TIME: 15:00
LOCAL: Qualificação por acesso remoto
TITLE:

Study of the healing activity of silk fibroin hydrogel loaded with extract of Bixa orellana L. (Bixaceae) seeds

KEY WORDS:

silk fibroin; annatto; wound repair; hydrogels; dressings.

PAGES: 118
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUMMARY:

Introduction: The high complexity and high costs of treatment make wound repair a challenge for the health sector, for patients and for society. In the last decade, there has been increasing progress in the development of biomaterials from natural polymers, such as silk fibroin, due to its unique wound healing properties. Bixa orellana seeds have therapeutic and pharmacological properties that contribute to the progress of the stages of the healing process. Wound care based on the use of silk fibroin-based hydrogels combined with the incorporation of active compounds from B. orellana seeds, aiming at controlling the microenvironment in wound healing, can provide better results. Objective: This project focuses on the development of a silk fibroin hydrogel loaded with the extract of B. orellana L. (Bixaceae) seeds and evaluation of the healing activity in vivo. Material and Methods: The project was divided into two stages. In stage I, extracts of B. orellana seeds were produced using a silk fibroin solution (SFS) instead of a solvent. Subsequently, the bixin content in the extracts was quantified by UV-Vis spectroscopy. The MTT assay was used to evaluate the cell viability of human dermal fibroblasts (HDFn) and macrophages (RAW 264.7) after exposure to the extracts. Stage II will consist of the development of the silk fibroin hydrogel (HFS), which will be obtained directly from the SFS dialysis process. ELSU + SFS (liquid extract of B. orellana seeds from the silk fibroin solution) will be incorporated into the HFS. After this, it will be initially characterized in terms of morphology (SEM), the presence of functional groups (FT-IR), thermal behavior (TGA, DTG and DSC) and rheological behavior. The preliminary stability of the hydrogel will then be assessed, starting with macroscopic analysis, centrifuge test, freeze-thaw cycle, determination of spreadability, viscosity and electrical conductivity, UV-Vis analysis, measurement of globule size and in vitro release tests. The assessment of hemocompatibility will be performed through the hemolysis test. While the antimicrobial potential will be assessed by the antimicrobial and antibiofilm tests. The healing potential will be assessed using the excisional skin wound model, with subsequent histopathological analysis to assess the effects of the hydrogel. Expected results: This project is expected to present an innovative, natural and sustainable therapeutic alternative for wound repair, and that the hydrogel produced can be studied as a bioactive dressing. Furthermore,
contribute to the advancement of research on silk fibroin-based biomaterials (I) obtained by environmentally friendly physical methods, (II) loaded with natural bioactive compounds and (III) with multifunctional properties for wound healing.

COMMITTEE MEMBERS:
Externo à Instituição - PABLO LUIS BAIA FIGUEIREDO - UEPA
Interno - 1924165 - FRANCISCO FABIO OLIVEIRA DE SOUSA
Presidente - 1867989 - IRLON MACIEL FERREIRA