Banca de QUALIFICAÇÃO: ANA BARBARA SOUZA VIANA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA BARBARA SOUZA VIANA
DATE: 19/10/2024
TIME: 09:00
LOCAL: Sala 1, Gerência de Ensino e Pesquisa do Hospital Universitário da UNIFAP
TITLE:

STUDY OF INJECTABLE NANOEMULSION OF β-CARYOPHYLENE ON DIABETIC ATHEROGENIC METABOLIC SYNDROME IN WISTAR RATS

KEY WORDS:

β-caryophyllene. nanotechnology, diabetes, atherosclerosis.

PAGES: 54
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUMMARY:

Introduction: β-Caryophyllene (BCP) is a widely studied sesquirterpene with modulatory, antioxidant and anti-inflammatory effects. The use of this compound has applicability through nanotechnology, in addition to its parenteral administration conferring effective and bioavailable therapeutic benefits. Objective: The objective of the present study is to develop an injectable BCP nanoemulsion and study its effects on atherogenic diabetic metabolic syndrome in Wistar rats. Material and Methods: Injectable BCP nanoemulsions were developed, followed by the evaluation of the acute toxicity and muscular compatibility of the injectable, evaluation of the antiatherosclerotic and antiglycemic activity of the nanoemulsion by induction of alloxan and saturated fat; biochemical analysis of the animals' serum; verification of the presence of atheroma in the animals' aortas by Scanning Electron Microscopy (SEM); biochemical analysis of the serum of the experimental animals; observation of possible histopathological changes in the liver and pancreas in animals with atherogenic diabetic metabolic syndrome (DMAS) after treatment with the nanoemulsion. Results and discussion: The formulations were analyzed for 28 days, showing some significant variations between their results both over the days and in the respective HLB value. The results of the last day showed that the formulations presented particle sizes ranging from 102.39±5.71 to 219.6±5.66nm, with a polydispersity index of 0.37±0.01 to 0.46±0.01 and zeta potential -27.5±0.6 to -13.10±0.36. Conclusion: The characterization and stability analysis performed for 28 days demonstrated that NBCP1, NBCP2, NBCP3 and NBCP4 presented particle sizes and polydispersity index with satisfactory results. However, after the physicochemical evaluation, the centrifugation test demonstrated instability in the system and phase separation. Therefore, it is concluded that NBCP1 presented the best result and stability within acceptable parameters.

COMMITTEE MEMBERS:
Externo ao Programa - 1031791 - HELISON DE OLIVEIRA CARVALHO - nullPresidente - 2509110 - JOSE CARLOS TAVARES CARVALHO